Second-site reversion of a structural defect in bacteriophage T4 lysozyme
Department of Molecular Genetics and Microbiology
Bacteriophage T4; Bacteriophage lambda; Base Sequence; Genetic Vectors; Models, Molecular; Molecular Sequence Data; Muramidase; Mutagenesis; Plasmids; Polymerase Chain Reaction; *Protein Folding; Selection (Genetics); Sequence Analysis, DNA
Life Sciences | Medicine and Health Sciences
A plasmid-borne gene encoding bacteriophage T4 lysozyme with a structural mutation, Tyr161-Ala, was mutagenized by by the use of polymerase chain reaction. The mutagenized gene was inserted into a specialized bacteriophage lambda cloning vector that must acquire a functional lysozyme gene in order to form plaques. Functional variants of the mutant lysozyme were selected. Three compensatory second-site revertants were obtained: Thr152-Met, Lys43-Ile, and Thr151-Ala. The effects of these mutations are interpreted in light of previous structural and genetic studies of T4 lysozyme.
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Citation: FASEB J. 1996 Jan;10(1):159-63.
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Bouvier, Suzanne E. and Poteete, Anthony R., "Second-site reversion of a structural defect in bacteriophage T4 lysozyme" (1996). Open Access Articles. 568.