UMMS Affiliation

Department of Cell Biology

Publication Date


Document Type



Aminohydrolases; Animals; Chlamydomonas reinhardtii; *Gene Silencing; *Promoter Regions (Genetics); RNA Interference; Trimethoprim-Sulfamethoxazole Combination


Cell Biology | Genetics and Genomics


An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.

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Citation: Eukaryot Cell. 2005 Nov;4(11):1959-62. Link to article on publisher's site

DOI of Published Version


Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Eukaryotic cell

PubMed ID




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