UMMS Affiliation

Department of Cell Biology

Date

11-10-2005

Document Type

Article

Subjects

Aminohydrolases; Animals; Chlamydomonas reinhardtii; *Gene Silencing; *Promoter Regions (Genetics); RNA Interference; Trimethoprim-Sulfamethoxazole Combination

Disciplines

Cell Biology | Genetics and Genomics

Abstract

An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.

Rights and Permissions

Citation: Eukaryot Cell. 2005 Nov;4(11):1959-62. Link to article on publisher's site

DOI of Published Version

10.1128/EC.4.11.1959-1962.2005

Related Resources

Link to Article in PubMed

Journal Title

Eukaryotic cell

PubMed ID

16278463

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