Department of Cell Biology
Aminohydrolases; Animals; Chlamydomonas reinhardtii; *Gene Silencing; *Promoter Regions (Genetics); RNA Interference; Trimethoprim-Sulfamethoxazole Combination
Cell Biology | Genetics and Genomics
An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.
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Citation: Eukaryot Cell. 2005 Nov;4(11):1959-62. Link to article on publisher's site
DOI of Published Version
Koblenz, Bettina and Lechtreck, Karl-Ferdinand, "The NIT1 promoter allows inducible and reversible silencing of centrin in Chlamydomonas reinhardtii" (2005). Open Access Articles. 550.