Etidronate inhibits the thyroid hormone-induced bone loss in rats assessed by bone mineral density and messenger ribonucleic acid markers of osteoblast and osteoclast function
Authors
Ongphiphadhanakul, BoonsongJenis, Louis G.
Braverman, Lewis E.
Alex, Sharon
Stein, Gary S.
Lian, Jane B.
Baran, Daniel T.
Document Type
Journal ArticlePublication Date
1993-12-01Keywords
AnimalsBiological Markers
Bone Density
Etidronic Acid
Femur
Male
Osteoblasts
Osteoclasts
Osteoporosis
RNA, Messenger
RNA, Ribosomal, 28S
Rats
Spine
Thyroxine
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
TSH-suppressive doses of thyroid hormone are associated with bone loss. We have previously reported that L-T4 decreases femoral, but not vertebral bone mineral density (BMD) in rats. As bisphosphonates are able to decrease bone resorption, especially in high bone turnover states, we investigated the potential effects of etidronate disodium (EHDP) on L-T4-induced bone loss in the rat model by assessing BMD and gene expression of osteoblast (osteocalcin, osteopontin, type I collagen, and alkaline phosphatase), osteoclast (tartrate-resistant acid phosphatase), and cell growth (histone) markers in the skeleton. L-T4 administered for 20 days decreased BMD in the femur, but had no effect on the lumbar spine. EHDP alone had no effect on femoral or vertebral BMD, but did prevent the L-T4-induced bone loss in the femur. L-T4 increased mRNA levels of alkaline phosphatase, tartrate-resistant acid phosphatase, and histone H4 in the femur, but not in the vertebrae. EHDP, which alone had no effect on gene expression in the femur or vertebrae, inhibited the effect of L-T4 on mRNA markers in the femur. The results demonstrate that EHDP can prevent the L-T4-induced decrease in femoral BMD in rats that is associated with the prevention of changes in mRNA markers of osteoclast and osteoblast function. EHDP and other bisphosphonate compounds may be useful in the prevention of thyroid hormone-induced bone loss in humans.Source
Endocrinology. 1993 Dec;133(6):2502-7.