Title

Cytoplasmic polyadenylation elements mediate masking and unmasking of cyclin B1 mRNA

UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date

4-16-1999

Document Type

Article

Subjects

3' Untranslated Regions; Animals; Base Sequence; Chloramphenicol O-Acetyltransferase; Cyclin B; Cytoplasm; DNA Primers; Oocytes; Poly A; Protein Biosynthesis; RNA, Messenger; Xenopus laevis

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

During oocyte maturation, cyclin B1 mRNA is translationally activated by cytoplasmic polyadenylation. This process is dependent on cytoplasmic polyadenylation elements (CPEs) in the 3' untranslated region (UTR) of the mRNA. To determine whether a titratable factor might be involved in the initial translational repression (masking) of this mRNA, high levels of cyclin B1 3' UTR were injected into oocytes. While this treatment had no effect on the poly(A) tail length of endogenous cyclin B1 mRNA, it induced cyclin B1 synthesis. A mutational analysis revealed that the most efficient unmasking element in the cyclin 3' UTR was the CPE. However, other U-rich sequences that resemble the CPE in structure, but which do not bind the CPE-binding polyadenylation factor CPEB, failed to induce unmasking. When fused to the chloramphenical acetyl transferase (CAT) coding region, the cyclin B1 3' UTR inhibited CAT translation in injected oocytes. In addition, a synthetic 3' UTR containing multiple copies of the CPE also inhibited translation, and did so in a dose-dependent manner. Furthermore, efficient CPE-mediated masking required cap-dependent translation. During the normal course of progesterone-induced maturation, cytoplasmic polyadenylation was necessary for mRNA unmasking. A model to explain how cyclin B1 mRNA masking and unmasking could be regulated by the CPE is presented.

Rights and Permissions

Citation: EMBO J. 1999 Apr 15;18(8):2294-303. Link to article on publisher's site

DOI of Published Version

10.1093/emboj/18.8.2294

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

The EMBO journal

PubMed ID

10205182