Title

Differential mRNA translation and meiotic progression require Cdc2-mediated CPEB destruction

UMMS Affiliation

Program in Molecular Medicine; Department of Molecular Genetics and Microbiology

Date

4-3-2002

Document Type

Article

Subjects

Amino Acid Sequence; Animals; CDC2 Protein Kinase; Cell Division; Humans; Meiosis; Metaphase; Molecular Sequence Data; Phosphorylation; Poly A; *Protein Biosynthesis; *RNA, Messenger; RNA-Binding Proteins; Transcription Factors; Ubiquitins; Xenopus; *Xenopus Proteins; *Zinc Fingers; *mRNA Cleavage and Polyadenylation Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Translational activation of several dormant mRNAs in vertebrate oocytes is mediated by cytoplasmic polyadenylation, a process controlled by the cytoplasmic polyadenylation element (CPE) and its binding protein CPEB. The translation of CPE-containing mRNAs does not occur en masse at any one time, but instead is temporally regulated. We show here that in Xenopus, partial destruction of CPEB controls the temporal translation of CPE-containing mRNAs. While some mRNAs, such as the one encoding Mos, are polyadenylated at prophase I, the polyadenylation of cyclin B1 mRNA requires the partial destruction of CPEB that occurs at metaphase I. CPEB destruction is mediated by a PEST box and Cdc2-catalyzed phosphorylation, and is essential for meiotic progression to metaphase II. CPEB destruction is also necessary for mitosis in the early embryo. These data indicate that a change in the CPEB:CPE ratio is necessary to activate mRNAs at metaphase I and drive the cells' entry into metaphase II.

Rights and Permissions

Citation: EMBO J. 2002 Apr 2;21(7):1833-44. Link to article on publisher's site

DOI of Published Version

10.1093/emboj/21.7.1833

Related Resources

Link to Article in PubMed

Journal Title

The EMBO journal

PubMed ID

11927567