UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Molecular Medicine

Date

7-1-2005

Document Type

Article

Subjects

Amino Acid Sequence; Animals; Body Patterning; Drosophila Proteins; Drosophila melanogaster; Embryo, Nonmammalian; Gastrula; Intracellular Signaling Peptides and Proteins; Molecular Sequence Data; Morphogenesis; Nuclear Proteins; Phosphoproteins; Sequence Alignment; Sequence Homology, Amino Acid; Transcription Factors; Twist Transcription Factor; Zinc Fingers

Disciplines

Biochemistry, Biophysics, and Structural Biology | Molecular Biology

Abstract

The maternal Toll signaling pathway sets up a nuclear gradient of the transcription factor Dorsal in the early Drosophila embryo. Dorsal activates twist and snail, and the Dorsal/Twist/Snail network activates and represses other zygotic genes to form the correct expression patterns along the dorsoventral axis. An essential function of this patterning is to promote ventral cell invagination during mesoderm formation, but how the downstream genes regulate ventral invagination is not known. We show here that wntD is a novel member of the Wnt family. The expression of wntD is activated by Dorsal and Twist, but the expression is much reduced in the ventral cells through repression by Snail. Overexpression of WntD in the early embryo inhibits ventral invagination, suggesting that the de-repressed WntD in snail mutant embryos may contribute to inhibiting ventral invagination. The overexpressed WntD inhibits invagination by antagonizing Dorsal nuclear localization, as well as twist and snail expression. Consistent with the early expression of WntD at the poles in wild-type embryos, loss of WntD leads to posterior expansion of nuclear Dorsal and snail expression, demonstrating that physiological levels of WntD can also attenuate Dorsal nuclear localization. We also show that the de-repressed WntD in snail mutant embryos contributes to the premature loss of snail expression, probably by inhibiting Dorsal. Thus, these results together demonstrate that WntD is regulated by the Dorsal/Twist/Snail network, and is an inhibitor of Dorsal nuclear localization and function.

Rights and Permissions

Citation: Development. 2005 Aug;132(15):3419-29. Epub 2005 Jun 29. Link to article on publisher's site

DOI of Published Version

10.1242/dev.01903

Related Resources

Link to Article in PubMed

Journal Title

Development (Cambridge, England)

PubMed ID

15987775

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