Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation
Department of Cell Biology
Animals; Bone Development; Cell Differentiation; Cell Division; Gene Expression; Gene Expression Regulation; Humans; Osteoblasts; Osteogenesis; Phenotype; Proteins
Life Sciences | Medicine and Health Sciences
The combined application of molecular, biochemical, histochemical, and ultrastructural approaches has defined a temporal sequence of gene expression associated with development of the bone cell phenotype in primary osteoblast cultures. The peak levels of expressed genes reflect a developmental sequence of bone cell differentiation characterized by three principal periods: proliferation, extracellular matrix maturation and mineralization, and two restriction points to which the cells can progress but cannot pass without further signals. The regulation of cell growth and bone-specific gene expression has been examined during this developmental sequence and is discussed within the context of several unique concepts. These are (1) that oncogene expression in proliferating osteoblasts contributes to the suppression of genes expressed postproliferatively, (2) that hormone modulation of a gene is dependent upon the maturational state of the osteoblast, and (3) that chromatin structure and the presence of nucleosomes contribute to three-dimensional organization of gene promoters that support synergistic and/or antagonistic activities of physiologic mediators of bone cell growth and differentiation.
Rights and Permissions
Citation: Crit Rev Oral Biol Med. 1992;3(3):269-305.
Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists
Lian, Jane B. and Stein, Gary S., "Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation" (1992). Open Access Articles. 469.