Multimodal quantitative imaging of brain cancer in cultured cells
Authors
Feng, XinMuzikansky, Alona
Ross, Alonzo H.
Hamblin, Michael R.
Jermain, Peter R.
Yaroslavsky, Anna N.
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
Journal ArticlePublication Date
2019-07-29Keywords
brain cancerglioblastoma cells
cancer cells
detection of brain cancer
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Bioimaging and Biomedical Optics
Cancer Biology
Cell Biology
Cells
Neoplasms
Metadata
Show full item recordAbstract
Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.Source
Biomed Opt Express. 2019 Jul 29;10(8):4237-4248. doi: 10.1364/BOE.10.004237. eCollection 2019 Aug 1. Link to article on publisher's site
DOI
10.1364/BOE.10.004237Permanent Link to this Item
http://hdl.handle.net/20.500.14038/41150PubMed ID
31453007Related Resources
Rights
Copyright © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement, https://www.osapublishing.org/submit/review/pdf/OSACopyTransferOAAgrmnt(2017-09-05).pdf.ae974a485f413a2113503eed53cd6c53
10.1364/BOE.10.004237
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