Department of Medicine; Department of Molecular Genetics and Microbiology
Alkylating Agents; Cisplatin; DNA Repair; DNA, Bacterial; Escherichia coli; Gene Expression Regulation
Life Sciences | Medicine and Health Sciences
Previous work has demonstrated heterogeneous effects of methylating agents on induction of DNA damage inducible genes in Escherichia coli. These studies employed E. coli mutants that have fusions of the lac operon to genes induced by treatment with sublethal levels of alkylating agents. These mutants were selected from random insertions of the Mu-dl (Apr lac) phage by screening for induction of beta-galactosidase activity in the presence of methylmethanesulfonate or N-methyl-N'-nitro-N-nitrosoguanidine. The current report extends these findings by analyzing gene expression caused by mechlorethamine, chloroethylnitrosoureas and cis-diamminedichloroplatinum(II) (cis-DDP). The results demonstrate heterogeneous effects by these agents on gene expression. While 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea induces alkA, other nitrosoureas, mechlorethamine, and cis-DDP do not cause expression of this gene. Further, while all nitrosoureas caused expression of aidC, mechlorethamine and cis-DDP did not. Lastly, cis-DDP caused marked expression of a sulA fusion mutant while not inducing any of the other E. coli fusion mutants.
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Citation: Cancer Res. 1988 Sep 1;48(17):4823-6.
Fram, Robert J.; Crockett, Jay; and Volkert, Michael R., "Gene expression caused by alkylating agents and cis-diamminedichloroplatinum(II) in Escherichia coli" (1988). Open Access Articles. 379.