UMMS Affiliation

Department of Cell Biology

Date

1-1-1994

Document Type

Article

Subjects

Animals; Antigens, Nuclear; Cell Differentiation; Female; Gene Expression Regulation, Neoplastic; Nuclear Proteins; Osteoblasts; Osteosarcoma; Phenotype; Pregnancy; Rats; Rats, Sprague-Dawley; Tumor Cells, Cultured; Tumor Markers, Biological

Disciplines

Cancer Biology | Cell Biology | Genetics and Genomics

Abstract

Interrelationships between nuclear architecture and gene expression were examined by comparing the representation of nuclear matrix proteins in ROS 17/2.8 rat and MG-63 human osteosarcoma cells with those in normal diploid osteoblasts. The tumor-derived cells coexpress genes which are expressed in a sequential and mutually exclusive manner during the progressive stages of osteoblast differentiation. In osteosarcoma cells two-dimensional electrophoretic analysis indicates a composite representation of nuclear matrix proteins characteristic of both the proliferative and postproliferative periods of osteoblast phenotype development. In addition, nuclear matrix proteins unique to the tumor cells and the absence of nuclear matrix proteins found only in normal diploid osteoblasts are observed. Tumor-specific nuclear matrix proteins include those expressed in a proliferation-dependent and independent manner. There is a parallel relationship between nuclear matrix proteins and the expression of cell growth and tissue-specific genes during osteoblast differentiation and in osteosarcoma cells where the developmental sequence of gene expression has been abrogated. Nuclear matrix proteins therefore provide markers reflecting defined periods of bone cell differentiation and phenotypic characteristics of an osteosarcoma.

Rights and Permissions

Citation: Cancer Res. 1994 Jan 1;54(1):28-32.

Related Resources

Link to article in PubMed

Journal Title

Cancer research

PubMed ID

8261453

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.