Towards the reconstruction of central nervous system white matter using neural precursor cells
Department of Neurology; Department of Pathology; Department of Cell Biology
Animals; Astrocytes; Axons; Cells, Cultured; Central Nervous System; Corpus Striatum; Embryo, Mammalian; Epidermal Growth Factor; Female; Injections, Intraventricular; Lateral Ventricles; Male; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Neurologic Mutants; Myelin Basic Proteins; Myelin Sheath; Oligodendroglia; *Stem Cell Transplantation; Stem Cells
Life Sciences | Medicine and Health Sciences
Epidermal growth factor-responsive neural precursor cells were used as donor cells for transplantation into wild-type and myelin-deficient shiverer (shi) mice. The cells engrafted robustly within the CNS following intracerebroventricular and cisternal transplantation in neonatal mice. The cells adopted glial phenotypes, and some functioned as oligodendrocytes, producing myelin basic protein and morphologically normal internodal myelin sheaths. When individual shi mice received two transplants (on post-natal days 1 and 3), donor-derived cells disseminated widely and expressed myelin basic protein in central white matter tracts throughout the brain.
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Citation: Brain. 2001 Nov;124(Pt 11):2147-61.