Krill Oil-In-Water Emulsion Protects against Lipopolysaccharide-Induced Proinflammatory Activation of Macrophages In Vitro
UMass Chan Affiliations
Department of Medicine, Division of GastroenterologyDocument Type
Journal ArticlePublication Date
2017-03-15Keywords
LPScytokines
krill oil-in-water emulsion
omega-3 fatty acids
phospholipids
septic shock
Chemicals and Drugs
Medicinal Chemistry and Pharmaceutics
Pharmacy and Pharmaceutical Sciences
Metadata
Show full item recordAbstract
BACKGROUND: Parenteral nutrition is often a mandatory therapeutic strategy for cases of septicemia. Likewise, therapeutic application of anti-oxidants, anti-inflammatory therapy, and endotoxin lowering, by removal or inactivation, might be beneficial to ameliorate the systemic inflammatory response during the acute phases of critical illness. Concerning anti-inflammatory properties in this setting, omega-3 fatty acids of marine origin have been frequently described. This study investigated the anti-inflammatory and LPS-inactivating properties of krill oil (KO)-in-water emulsion in human macrophages in vitro. MATERIALS AND METHODS: Differentiated THP-1 macrophages were activated using specific ultrapure-LPS that binds only on the toll-like receptor 4 (TLR4) in order to determine the inhibitory properties of the KO emulsion on the LPS-binding capacity, and the subsequent release of TNF-alpha. RESULTS: KO emulsion inhibited the macrophage binding of LPS to the TLR4 by 50% (at 12.5 microg/mL) and 75% (at 25 microg/mL), whereas, at 50 microg/mL, completely abolished the LPS binding. Moreover, KO (12.5 microg/mL, 25 microg/mL, or 50 microg/mL) also inhibited (30%, 40%, or 75%, respectively) the TNF-alpha release after activation with 0.01 microg/mL LPS in comparison with LPS treatment alone. CONCLUSION: KO emulsion influences the LPS-induced pro-inflammatory activation of macrophages, possibly due to inactivation of the LPS binding capacity.Source
Mar Drugs. 2017 Mar 15;15(3). pii: E74. doi: 10.3390/md15030074. Link to article on publisher's siteDOI
10.3390/md15030074Permanent Link to this Item
http://hdl.handle.net/20.500.14038/40315PubMed ID
28294970Related Resources
Rights
Copyright © 2017 by the authors.Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.3390/md15030074