Department of Pediatrics; Division of Hematology and Oncology
Antibodies, Monoclonal; B-Lymphocytes; Binding, Competitive; Cell Line, Transformed; Cytotoxicity Tests, Immunologic; Genetic Vectors; HIV Seropositivity; HIV-1; Hemophilia A; Histocompatibility Antigens Class I; Humans; Immunity, Cellular; Killer Cells, Natural; Prospective Studies; T-Lymphocytes, Cytotoxic
Hematology | Oncology | Pediatrics | Virus Diseases
Major histocompatibility (MHC)-restricted, human immunodeficiency virus type one (HIV-1)-specific, cytotoxic T lymphocytes (CTLs) were detected in the peripheral blood mononuclear cells (PBMCs) of HIV-1-infected individuals. Using a system of autologous B and T lymphoblastoid cell lines infected with recombinant vaccinia vectors (VVs) expressing HIV-1 gene products, we were able to detect HIV-1-specific cytolytic responses in the PBMCs of 88% of HIV-1-seropositive hemophiliac patients in the absence of in vitro stimulation. These cytolytic responses were directed against both HIV-1 envelope and gag gene products. The responses were resistant to natural killer (NK) cell depletion and were inhibited by monoclonal antibodies (MoAbs) to the T cell receptor, CD8 surface antigens, and MHC class I antigens, suggesting a classical MHC class I restricted, virus-specific CTL response.
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Citation: Blood. 1989 May 15;73(7):1909-14.
Koup, Richard A.; Sullivan, John L.; Levine, Peter H.; Brettler, Doreen B.; Mahr, Anna; Mazzara, Gail; McKenzie, Sara; and Panicali, Dennis, "Detection of major histocompatibility complex class I-restricted, HIV-specific cytotoxic T lymphocytes in the blood of infected hemophiliacs" (1989). Open Access Articles. 309.