UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology

Publication Date

2-28-2017

Document Type

Article

Disciplines

Genetics and Genomics | Immunology and Infectious Disease | Parasitic Diseases

Abstract

BACKGROUND: Clinical manifestations of cutaneous leishmaniasis (CL) caused by Leishmania (Viannia) range from asymptomatic infection to self-limited, or chronic (non-healing) cutaneous lesions. Given the critical role of the immune response in the clinical outcome of CL, it is plausible that functional polymorphisms in immune-related genes contribute to define the clinical manifestations of human infection.

METHODS: DNA samples from a retrospective cohort of individuals from an endemic area of L. V. panamensis transmission in Colombia were used to determine the frequency of SNPs in TNFalpha, IL-10 and TLR4 genes. DNA samples were obtained from 74 adult participants: 38 patients presenting chronic cutaneous leishmaniasis (CCL) and 36 individuals with asymptomatic infection. Genotyping of TNFalpha-308G/A, IL-10-819C/T, and TLR4 Asp299Gly and Thr399Ile SNPs, was conducted by PCR-restriction fragment length polymorphisms. Allele, genotype frequencies and associations between SNPs and clinical groups were evaluated.

RESULTS: The A allele in TNFalpha-308G/A SNP was found more frequently in individuals with asymptomatic infection (16% vs 7%), whereas the CC genotype in IL-10-819 C/T SNP was more frequent in patients with CCL (34% vs. 27% in asymptomatic individuals). No differences in allele frequencies for TLR4 SNPs were found among groups.

CONCLUSION: This study provides a reference base for statistical power calculation and design of association studies of genetic polymorphisms in immune response related-genes and the pathogenesis of infections caused by L. V. panamensis.

Rights and Permissions

Copyright © The Author(s). 2017. Citation: BMC Infect Dis. 2017 Feb 28;17(1):177. doi: 10.1186/s12879-017-2281-4. Link to article on publisher's site

DOI of Published Version

10.1186/s12879-017-2281-4

Related Resources

Link to Article in PubMed

Keywords

symptomatic infection, chronicity, cutaneous leishmaniasis, single nucleotide polymorphism

Journal/Book/Conference Title

BMC infectious diseases

PubMed ID

28241747

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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