UMMS Affiliation

Department of Cell and Developmental Biology; Department of Radiology; Witman Lab

Date

3-14-2017

Document Type

Article

Disciplines

Cell Biology | Cellular and Molecular Physiology | Developmental Biology

Abstract

We have used an insertional mutagenesis approach to generate new C. reinhardtii motility mutants. Of 56 mutants isolated, one is a new allele at the ODA3 locus, called oda3-6. Similar to the previously characterized oda3 alleles, oda3-6 has a slow-jerky swimming phenotype and reduced swimming speed. The oda3-6 mutant fails to assemble the outer dynein arm motor and outer dynein arm-docking complex (ODA-DC) in the ciliary axoneme due to an insertion in the 5' end of the DCC1 gene, which encodes the DC1 subunit of the ODA-DC. Transformation of oda3-6 with the wild-type DCC1 gene rescues the mutant swimming phenotype and restores assembly of the ODA-DC and the outer dynein arm in the cilium. This is the first oda3 mutant to be characterized at the molecular level and is likely to be very useful for further analysis of DC1 function.

Rights and Permissions

Copyright: © 2017 Brown et al. Citation: PLoS One. 2017 Mar 14;12(3):e0173842. eCollection 2017. Link to article on publisher's site

DOI of Published Version

10.1371/journal.pone.0173842

Related Resources

Link to Article in PubMed

Journal Title

PloS one

PubMed ID

28291812

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.