UMMS Affiliation

Department of Pediatrics University of Massachusetts Medical School

Publication Date

11-15-1989

Document Type

Article

Subjects

Blotting, Western; Cell Differentiation; Cell Nucleus; Cycloheximide; Gene Expression Regulation, Leukemic; Glutathione Peroxidase; Humans; Leukemia, Myeloid; Protein Biosynthesis; RNA, Messenger; Selenium; Transcription, Genetic; Tumor Cells, Cultured

Disciplines

Cancer Biology | Cell and Developmental Biology | Hematology | Medical Genetics | Pediatrics

Abstract

We have used a cloned cDNA for the major human selenoprotein, glutathione peroxidase (GPx), to assess the mode of regulation of human GPx gene (GPX-1) expression by selenium. When the HL-60 human myeloid cell line is grown in a selenium-deficient medium, GPx enzymatic activity decreases 30-fold compared with selenium-replete cells. Upon return to a medium containing selenium in the form of selenite, GPx activity in the cells starts to increase within 48 hours and reaches maximal (selenium-replete) levels at 7 days. Steady-state immunoreactive protein levels correlate with enzymatic activity. Cycloheximide inhibits the rise in GPx activity that accompanies selenium replenishment, indicating that protein synthesis is required for the increase. However, GPx mRNA levels and the rate of transcription of the human GPx gene change very little and thus appear to be independent of the selenium supply. Thus the human GPx gene appears to be regulated post-transcriptionally, probably cotranslationally, in response to selenium availability.

Rights and Permissions

Citation: Blood. 1989 Nov 15;74(7):2535-41.

Related Resources

Link to article in PubMed

Journal/Book/Conference Title

Blood

PubMed ID

2804377

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