Title

Chromatin dynamics regulate mesenchymal stem cell lineage specification and differentiation to osteogenesis

UMMS Affiliation

Department of Cell and Developmental Biology; Department of Medicine

Publication Date

4-1-2017

Document Type

Article

Disciplines

Cell Biology | Developmental Biology

Abstract

Multipotent mesenchymal stromal cells (MSCs) are critical for regeneration of multiple tissues. Epigenetic mechanisms are fundamental regulators of lineage specification and cell fate, and as such, we addressed the question of which epigenetic modifications characterize the transition of nascent MSCs to a tissue specific MSC-derived phenotype. By profiling the temporal changes of seven histone marks correlated to gene expression during proliferation, early commitment, matrix deposition, and mineralization stages, we identified distinct epigenetic mechanisms that regulate transcriptional programs necessary for tissue-specific phenotype development. Patterns of stage-specific enrichment of histone modifications revealed distinct modes of repression and activation of gene expression that would not be detected using single endpoint analysis. We discovered that at commitment, H3K27me3 is removed from genes that are upregulated and is not acquired on downregulated genes. Additionally, we found that the absence of H3K4me3 modification at promoters defined a subset of osteoblast-specific upregulated genes, indicating that acquisition of acetyl modifications drive activation of these genes. Significantly, loss or gain of H3K36me3 was the primary predictor of dynamic changes in temporal gene expression. Using unsupervised pattern discovery analysis the signature of osteogenic-related histone modifications identified novel functional cis regulatory modules associated with enhancer regions that control tissue-specific genes. Our work provides a cornerstone to understand the epigenetic regulation of transcriptional programs that are important for MSC lineage commitment and lineage, as well as insights to facilitate MSC-based therapeutic interventions.

Rights and Permissions

Citation: Biochim Biophys Acta. 2017 Apr. Epub 2017 Jan 8; 1860(4):438-449. 10.1016/j.bbagrm.2017.01.003. Link to article on publisher's site

DOI of Published Version

10.1016/j.bbagrm.2017.01.003

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Biochimica et biophysica acta

PubMed ID

28077316