UMMS Affiliation

Department of Cell and Developmental Biology; Nickerson Lab

Date

12-1-2016

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Cell Biology

Abstract

Mechanical integration of the nucleus with the extracellular matrix (ECM) is established by linkage between the cytoskeleton and the nucleus. This integration is hypothesized to mediate sensing of ECM rigidity, but parsing the function of nucleus-cytoskeleton linkage from other mechanisms has remained a central challenge. Here we took advantage of the fact that the LINC (linker of nucleoskeleton and cytoskeleton) complex is a known molecular linker of the nucleus to the cytoskeleton, and asked how it regulates the sensitivity of genome-wide transcription to substratum rigidity. We show that gene mechanosensitivity is preserved after LINC disruption, but reversed in direction. Combined with myosin inhibition studies, we identify genes that depend on nuclear tension for their regulation. We also show that LINC disruption does not attenuate nuclear shape sensitivity to substrate rigidity. Our results show for the first time that the LINC complex facilitates mechano-regulation of expression across the genome.

Rights and Permissions

Copyright © 2016, The Author(s). Citation: Sci Rep. 2016 Dec 1;6:38063. doi: 10.1038/srep38063. Link to article on publisher's site

DOI of Published Version

10.1038/srep38063

Related Resources

Link to Article in PubMed

Keywords

Biophysics, Gene expression

Journal Title

Scientific reports

PubMed ID

27905489

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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