UMMS Affiliation

MassBiologics

Date

12-13-2016

Document Type

Article

Disciplines

Immunity | Immunoprophylaxis and Therapy

Abstract

Antibodies raised in Indian rhesus macaques [Macaca mulatta (MM)] in many preclinical vaccine studies are often evaluated in vitro for titer, antigen-recognition breadth, neutralization potency, and/or effector function, and in vivo for potential associations with protection. However, despite reliance on this key animal model in translation of promising candidate vaccines for evaluation in first in man studies, little is known about the properties of MM immunoglobulin G (IgG) subclasses and how they may compare to human IgG subclasses. Here, we evaluate the binding of MM IgG1, IgG2, IgG3, and IgG4 to human Fc gamma receptors (FcgammaR) and their ability to elicit the effector functions of human FcgammaR-bearing cells, and unlike in humans, find a notable absence of subclasses with dramatically silent Fc regions. Biophysical, in vitro, and in vivo characterization revealed MM IgG1 exhibited the greatest effector function activity followed by IgG2 and then IgG3/4. These findings in rhesus are in contrast with the canonical understanding that IgG1 and IgG3 dominate effector function in humans, indicating that subclass-switching profiles observed in rhesus studies may not strictly recapitulate those observed in human vaccine studies.

Rights and Permissions

Copyright © 2016 Boesch, Osei-Owusu, Crowley, Chu, Chan, Weiner, Bharadwaj, Hards, Adamo, Gerber, Cocklin, Schmitz, Miles, Eckman, Belli, Reimann and Ackerman. Citation: Front Immunol. 2016 Dec 13;7:589. ecollection 2016. Link to article on publisher's site

DOI of Published Version

10.3389/fimmu.2016.00589

Comments

Full author list omitted for brevity. For the full list of authors, see article.

Related Resources

Link to Article in PubMed

Keywords

Fc receptor, IgG, effector function, non-human primate, rhesus

Journal Title

Frontiers in immunology

PubMed ID

28018355

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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