UMMS Affiliation

Graduate School of Biomedical Sciences; School of Medicine

Publication Date

11-11-2016

Document Type

Article

Disciplines

Cancer Biology | Cell Biology

Abstract

BACKGROUND: The ability to transform normal human cells into cancer cells with the introduction of defined genetic alterations is a valuable method for understanding the mechanisms of oncogenesis. Easy establishment of immortalized but non-transformed human cells from various tissues would facilitate these genetic analyses.

RESULTS: We report here a simple, one-step immortalization method that involves retroviral vector mediated co-expression of the human telomerase protein and a shRNA targeting the CDKN2A gene locus. We demonstrate that this method could successfully immortalize human small airway epithelial cells while maintaining their chromosomal stability. We further showed that these cells retain p53 activity and can be transformed by the KRAS oncogene.

CONCLUSIONS: Our method simplifies the immortalization process and is broadly applicable for establishing immortalized epithelial cell lines from primary human tissues for cancer research.

Rights and Permissions

© The Author(s) 2016. Citation: Cell Biosci. 2016 Nov 11;6:57. eCollection 2016. Link to article on publisher's site

DOI of Published Version

10.1186/s13578-016-0122-6

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Cell and bioscience

PubMed ID

27891214

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.