Department of Molecular, Cell and Cancer Biology; Graduate School of Biomedical Sciences, Interdisciplinary Graduate Program
Chromatin assembly factor 1 (CAF-1) deposits histones during DNA synthesis. The p150 subunit of human CAF-1 contains an N-terminal domain (p150N) that is dispensable for histone deposition but promotes the localization of specific loci (nucleolar-associated domains [NADs]) and proteins to the nucleolus during interphase. One of the p150N-regulated proteins is proliferation antigen Ki-67, whose depletion also decreases the nucleolar association of NADs. Ki-67 is also a fundamental component of the perichromosomal layer (PCL), a sheath of proteins surrounding condensed chromosomes during mitosis. We show here that a subset of p150 localizes to the PCL during mitosis and that p150N is required for normal levels of Ki-67 accumulation on the PCL. This activity requires the sumoylation-interacting motif within p150N, which is also required for the nucleolar localization of NADs and Ki-67 during interphase. In this manner, p150N coordinates both interphase and mitotic nuclear structures via Ki67.
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Citation: Mol Biol Cell. 2017 Jan 1;28(1):21-29. doi: 10.1091/mbc.E16-09-0659. Epub 2016 Nov 2. Link to article on publisher's site. © 2017 Matheson and Kaufman. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
DOI of Published Version
Molecular biology of the cell
Matheson, Timothy D. and Kaufman, Paul D., "The p150N domain of chromatin assembly factor-1 regulates Ki-67 accumulation on the mitotic perichromosomal layer" (2017). Open Access Articles. 2975.
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This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.