UMMS Affiliation

Program in Molecular Medicine

Date

2-1-2017

Document Type

Article

Disciplines

Cell Biology | Cellular and Molecular Physiology

Abstract

Autophagy is an evolutionarily conserved intracellular system that maintains cellular homeostasis by degrading and recycling damaged cellular components. The transcription factor HLH-30/TFEB-mediated autophagy has been reported to regulate tolerance to bacterial infection, but less is known about the bona fide bacterial effector that activates HLH-30 and autophagy. Here, we reveal that bacterial membrane pore-forming toxin (PFT) induces autophagy in an HLH-30-dependent manner in Caenorhabditis elegans. Moreover, autophagy controls the susceptibility of animals to PFT toxicity through xenophagic degradation of PFT and repair of membrane-pore cell-autonomously in the PFT-targeted intestinal cells in C. elegans. These results demonstrate that autophagic pathways and autophagy are induced partly at the transcriptional level through HLH-30 activation and are required to protect metazoan upon PFT intoxication. Together, our data show a new and powerful connection between HLH-30-mediated autophagy and epithelium intrinsic cellular defense against the single most common mode of bacterial attack in vivo.

Rights and Permissions

Copyright © 2017 The Author(s). Published with license by Taylor & Francis. Citation: Autophagy. 2017 Feb;13(2):371-385. doi: 10.1080/15548627.2016.1256933. Epub 2016 Nov 22. Link to article on publisher's site

DOI of Published Version

10.1080/15548627.2016.1256933

Comments

Full author list omitted for brevity. For full list of authors see article.

Related Resources

Link to Article in PubMed

Keywords

C. elegans, HLH-30/TFEB, autophagy, effector triggered immunity (ETI), intrinsic cellular defense (INCED), pore-forming toxin (PFT), surveillance immunity

Journal Title

Autophagy

PubMed ID

27875098

Creative Commons License

Creative Commons Attribution-Noncommercial 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License

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