UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; UMass Metabolic Network

Publication Date

9-6-2016

Document Type

Article

Disciplines

Genomics | Structural Biology

Abstract

Chromatin is thought to carry epigenetic information from one generation to the next, although it is unclear how such information survives the disruptions of nucleosomal architecture occurring during genomic replication. Here, we measure a key aspect of chromatin structure dynamics during replication-how rapidly nucleosome positions are established on the newly replicated daughter genomes. By isolating newly synthesized DNA marked with 5-ethynyl-2'-deoxyuridine (EdU), we characterize nucleosome positions on both daughter genomes of S. cerevisiae during chromatin maturation. We find that nucleosomes rapidly adopt their mid-log positions at highly transcribed genes, which is consistent with a role for transcription in positioning nucleosomes in vivo. Additionally, experiments in hir1Delta mutants reveal a role for HIR in nucleosome spacing. We also characterized nucleosome positions on the leading and lagging strands, uncovering differences in chromatin maturation dynamics at hundreds of genes. Our data define the maturation dynamics of newly replicated chromatin and support a role for transcription in sculpting the chromatin template.

Rights and Permissions

Citation: Cell Rep. 2016 Sep 6;16(10):2651-65. doi: 10.1016/j.celrep.2016.07.083. Epub 2016 Aug 25. Link to article on publisher's site. Open Access funded by European Research Council.

DOI of Published Version

10.1016/j.celrep.2016.07.083

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Cell reports

PubMed ID

27568571

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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