UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

5-31-2016

Document Type

Article

Disciplines

Biochemistry | Biophysics | Structural Biology

Abstract

The spliceosome is a complex machine composed of small nuclear ribonucleoproteins (snRNPs) and accessory proteins that excises introns from pre-mRNAs. After assembly the spliceosome is activated for catalysis by rearrangement of subunits to form an active site. How this rearrangement is coordinated is not well-understood. During activation, U4 must be released to allow U6 conformational change, while Prp19 complex (NTC) recruitment is essential for stabilizing the active site. We used multi-wavelength colocalization single molecule spectroscopy to directly observe the key events in Saccharomyces cerevisiae spliceosome activation. Following binding of the U4/U6.U5 tri-snRNP, the spliceosome either reverses assembly by discarding tri-snRNP or proceeds to activation by irreversible U4 loss. The major pathway for NTC recruitment occurs after U4 release. ATP stimulates both the competing U4 release and tri-snRNP discard processes. The data reveal the activation mechanism and show that overall splicing efficiency may be maintained through repeated rounds of disassembly and tri-snRNP reassociation.

Rights and Permissions

Citation: Elife. 2016 May 31;5. pii: e14166. doi: 10.7554/eLife.14166. Link to article on publisher's site

Copyright © 2016, Hoskins et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

DOI of Published Version

10.7554/eLife.14166

Related Resources

Link to Article in PubMed

Keywords

RNA, S. cerevisiae, biochemistry, biophysics, fluorescence, single-molecule, snRNP, spliceosome, splicing, structural biology

Journal Title

eLife

PubMed ID

27244240

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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