UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology

Publication Date

7-12-2016

Document Type

Article

Disciplines

Immunity | Immunology of Infectious Disease | Immunopathology

Abstract

Inflammasome activation is associated with numerous diseases. However, in vivo detection of the activated inflammasome complex has been limited by a dearth of tools. We have developed transgenic mice that ectopically express the fluorescent adaptor protein, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and characterized the formation of assembled inflammasome complexes ("specks") in primary cells and tissues. In addition to hematopoietic cells, we have found that a stromal population in the lung tissues formed specks during the early phase of influenza infection, whereas myeloid cells showed speck formation after 2 days. In a peritonitis and group B streptococcus infection model, a higher percentage of neutrophils formed specks at early phases of infection, while dendritic cells formed specks at later time points. Furthermore, speck-forming cells underwent pyroptosis and extensive release of specks to the extracellular milieu in vivo. These data underscore the importance of free specks during inflammatory processes in vivo.

Rights and Permissions

Citation: Cell Rep. 2016 Jul 12;16(2):571-82. doi: 10.1016/j.celrep.2016.06.011. Epub 2016 Jun 23. Link to article on publisher's site

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

DOI of Published Version

10.1016/j.celrep.2016.06.011

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Cell reports

PubMed ID

27346360

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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