UMMS Affiliation

Department of Neurobiology; Hong-Sheng Li Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

3-8-2016

Document Type

Article

Disciplines

Molecular and Cellular Neuroscience

Abstract

Recycling of neurotransmitters is essential for sustained neuronal signaling, yet recycling pathways for various transmitters, including histamine, remain poorly understood. In the first visual ganglion (lamina) of Drosophila, photoreceptor-released histamine is taken up into perisynaptic glia, converted to carcinine, and delivered back to the photoreceptor for histamine regeneration. Here, we identify an organic cation transporter, CarT (carcinine transporter), that transports carcinine into photoreceptors during histamine recycling. CarT mediated in vitro uptake of carcinine. Deletion of the CarT gene caused an accumulation of carcinine in laminar glia accompanied by a reduction in histamine, resulting in abolished photoreceptor signal transmission and blindness in behavioral assays. These defects were rescued by expression of CarT cDNA in photoreceptors, and they were reproduced by photoreceptor-specific CarT knockdown. Our findings suggest a common role for the conserved family of CarT-like transporters in maintaining histamine homeostasis in both mammalian and fly brains.

Rights and Permissions

Citation: Cell Rep. 2016 Mar 8;14(9):2076-83. doi: 10.1016/j.celrep.2016.02.009. Epub 2016 Feb 25. Link to article on publisher's site

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

DOI of Published Version

10.1016/j.celrep.2016.02.009

Comments

Co-author Peiyi Guo is a doctoral student in the Neuroscience Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources

Link to Article in PubMed

Keywords

histamine, membrane transporter, neurotransmitter recycling, vision

Journal Title

Cell reports

PubMed ID

26923590

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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