UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Department of Medicine, Division of Hematology and Oncology

Publication Date

11-17-2015

Document Type

Article

Disciplines

Biochemistry

Abstract

RBM15, an RNA binding protein, determines cell-fate specification of many tissues including blood. We demonstrate that RBM15 is methylated by protein arginine methyltransferase 1 (PRMT1) at residue R578 leading to its degradation via ubiquitylation by an E3 ligase (CNOT4). Overexpression of PRMT1 in acute megakaryocytic leukemia cell lines blocks megakaryocyte terminal differentiation by downregulation of RBM15 protein level. Restoring RBM15 protein level rescues megakaryocyte terminal differentiation blocked by PRMT1 overexpression. At the molecular level, RBM15 binds to pre-mRNA intronic regions of genes important for megakaryopoiesis such as GATA1, RUNX1, TAL1 and c-MPL. Furthermore, preferential binding of RBM15 to specific intronic regions recruits the splicing factor SF3B1 to the same sites for alternative splicing. Therefore, PRMT1 regulates alternative RNA splicing via reducing RBM15 protein concentration. Targeting PRMT1 may be a curative therapy to restore megakaryocyte differentiation for acute megakaryocytic leukemia.

Rights and Permissions

Citation: Elife. 2015 Nov 17;4. pii: e07938. doi: 10.7554/eLife.07938. Link to article on publisher's site

DOI of Published Version

10.7554/eLife.07938

Comments

© 2015, Zhang et al. This article is distributed under the terms of theCreative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Related Resources

Link to Article in PubMed

Keywords

biochemistry, human, human biology, medicine

Journal/Book/Conference Title

eLife

PubMed ID

26575292

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Included in

Biochemistry Commons

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