Department of Biochemistry and Molecular Pharmacology
Biochemistry | Cell Biology | Genetics
The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed "Opto-CRAC") that selectively and remotely controls Ca2+ oscillations and Ca2+-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca2+-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded "photoactivatable adjuvant" to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote control of Ca2+-modulated activities with tailored function.
Rights and Permissions
Citation: Elife. 2015 Dec 8;4. pii: e10024. doi: 10.7554/eLife.10024. Link to article on publisher's site
DOI of Published Version
Calcium signaling, Immune response, Nanoparticles, Near infrared, Optogenetics, STIM1, biochemistry, cell biology, human, mouse
He, Lian; Zhang, Yuanwei; Ma, Guolin; Tan, Peng; Li, Zhan Jun; Zhang, Shengbing; Wu, Xiang; Jing, Ji; Fang, Shaohai; Zhou, Lijuan; Wang, Youjun; Huang, Yun; Hogan, Patrick; Han, Gang; and Zhou, Yubin, "Near-infrared photoactivatable control of Ca signaling and optogenetic immunomodulation" (2015). Open Access Articles. 2645.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.