UMMS Affiliation

Department of Neurobiology; Alkema Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

9-8-2015

Document Type

Article

Disciplines

Behavioral Neurobiology

Abstract

Behavioral output of neural networks depends on a delicate balance between excitatory and inhibitory synaptic connections. However, it is not known whether network formation and stability is constrained by the sign of synaptic connections between neurons within the network. Here we show that switching the sign of a synapse within a neural circuit can reverse the behavioral output. The inhibitory tyramine-gated chloride channel, LGC-55, induces head relaxation and inhibits forward locomotion during the Caenorhabditis elegans escape response. We switched the ion selectivity of an inhibitory LGC-55 anion channel to an excitatory LGC-55 cation channel. The engineered cation channel is properly trafficked in the native neural circuit and results in behavioral responses that are opposite to those produced by activation of the LGC-55 anion channel. Our findings indicate that switches in ion selectivity of ligand-gated ion channels (LGICs) do not affect network connectivity or stability and may provide an evolutionary and a synthetic mechanism to change behavior.

Rights and Permissions

Citation: PLoS Biol. 2015 Sep 8;13(9):e1002238. doi: 10.1371/journal.pbio.1002238. eCollection 2015.Link to article on publisher's site

DOI of Published Version

10.1371/journal.pbio.1002238

Comments

First author Jennifer Pirri is a doctoral student in the Neuroscience Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources

Link to Article in PubMed

Journal Title

PLoS biology

PubMed ID

26348462

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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