RNA Therapeutics Institute; Department of Biochemistry and Molecular Pharmacology
Biophysics | Molecular Biology
Imaging single molecules in live cells in 4+ D (space, time and colors) is crucial for studying various biological processes, especially for observing the behavior of RNA molecules within the nuclear landscape . RNA molecules are known to serve a multitude of tasks such as being templates for protein translation or to act as enzymes for regulating countless reactions in the nucleus . Studying RNA kinetics in living cells can provide new information on RNA function or even human diseases, for instance caused by viruses such as the human immunodeficiency virus (HIV) . A challenge to imaging nuclear RNA function is that the nucleus as a whole undergoes major reformation during the cell cycle  but the time required to step through the sample limits the capability to image large numbers of rapidly moving particles in a 3D space.
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Citation: Oncotarget. 2015 Oct 6;6(30):28515-6. doi: 10.18632/oncotarget.5121. Link to article on publisher's site
DOI of Published Version
3D microscopy, biophysics, mRNA trafficking, single molecule imaging
Smith, Carlas; Tu, Li-Chun; and Grünwald, David, "A 4D view on mRNA" (2015). Open Access Articles. 2594.
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