UMMS Affiliation

RNA Therapeutics Institute; Department of Biochemistry and Molecular Pharmacology

Date

10-6-2015

Document Type

Editorial

Disciplines

Biophysics | Molecular Biology

Abstract

Imaging single molecules in live cells in 4+ D (space, time and colors) is crucial for studying various biological processes, especially for observing the behavior of RNA molecules within the nuclear landscape [1]. RNA molecules are known to serve a multitude of tasks such as being templates for protein translation or to act as enzymes for regulating countless reactions in the nucleus [1]. Studying RNA kinetics in living cells can provide new information on RNA function or even human diseases, for instance caused by viruses such as the human immunodeficiency virus (HIV) [2]. A challenge to imaging nuclear RNA function is that the nucleus as a whole undergoes major reformation during the cell cycle [1] but the time required to step through the sample limits the capability to image large numbers of rapidly moving particles in a 3D space.

Rights and Permissions

Citation: Oncotarget. 2015 Oct 6;6(30):28515-6. doi: 10.18632/oncotarget.5121. Link to article on publisher's site

DOI of Published Version

10.18632/oncotarget.5121

Comments

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.

Related Resources

Link to Article in PubMed

Keywords

3D microscopy, biophysics, mRNA trafficking, single molecule imaging

Journal Title

Oncotarget

PubMed ID

26387129

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

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