UMMS Affiliation

Department of Molecular, Cell, and Cancer Biology; Program in Molecular Medicine

Date

10-6-2015

Document Type

Article

Disciplines

Biophysics | Cell Biology | Genomics | Molecular Biology | Structural Biology

Abstract

Functional interactions between gene regulatory factors and chromatin architecture have been difficult to directly assess. Here, we use micrococcal nuclease (MNase) footprinting to probe the functions of two chromatin-remodeling complexes. By simultaneously quantifying alterations in small MNase footprints over the binding sites of 30 regulatory factors in mouse embryonic stem cells (ESCs), we provide evidence that esBAF and Mbd3/NuRD modulate the binding of several regulatory proteins. In addition, we find that nucleosome occupancy is reduced at specific loci in favor of subnucleosomes upon depletion of esBAF, including sites of histone H2A.Z localization. Consistent with these data, we demonstrate that esBAF is required for normal H2A.Z localization in ESCs, suggesting esBAF either stabilizes H2A.Z-containing nucleosomes or promotes subnucleosome to nucleosome conversion by facilitating H2A.Z deposition. Therefore, integrative examination of MNase footprints reveals insights into nucleosome dynamics and functional interactions between chromatin structure and key gene-regulatory factors.

Rights and Permissions

Citation: Cell Rep. 2015 Oct 6;13(1):61-9. doi: 10.1016/j.celrep.2015.08.071. Epub 2015 Sep 24. Link to article on publisher's site

DOI of Published Version

10.1016/j.celrep.2015.08.071

Comments

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Related Resources

Link to Article in PubMed

Journal Title

Cell reports

PubMed ID

26411677

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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