Department of Neurology
Cell Biology | Developmental Biology | Genetics and Genomics | Neurology
Intragenic microRNAs (miRNAs), located mostly in the introns of protein-coding genes, are often co-expressed with their host mRNAs. However, their functional interaction in development is largely unknown. Here we show that in Drosophila, miR-92a and miR-92b are embedded in the intron and 3'UTR of jigr1, respectively, and co-expressed with some jigr1 isoforms. miR-92a and miR-92b are highly expressed in neuroblasts of larval brain where Jigr1 expression is low. Genetic deletion of both miR-92a and miR-92b demonstrates an essential cell-autonomous role for these miRNAs in maintaining neuroblast self-renewal through inhibiting premature differentiation. We also show that miR-92a and miR-92b directly target jigr1 in vivo and that some phenotypes due to the absence of these miRNAs are partially rescued by reducing the level of jigr1. These results reveal a novel function of the miR-92 family in Drosophila neuroblasts and provide another example that local negative feedback regulation of host genes by intragenic miRNAs is essential for animal development.
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Citation: PLoS Genet. 2015 May 22;11(5):e1005264. doi: 10.1371/journal.pgen.1005264. eCollection 2015. Link to article on publisher's site
DOI of Published Version
Yuva-Aydemir, Yeliz; Xu, Xia-Lian; Aydemir, Ozkan; Gascon, Eduardo; Sayin, Serkan; Zhou, Wenke; Hong, Yang; and Gao, Fen-Biao, "Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila" (2015). Open Access Articles. 2532.
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