Dengue viral RNA levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status
Authors
Srikiatkhachorn, AnonWichit, Sineewanlaya
Gibbons, Robert V.
Green, Sharone
Libraty, Daniel H.
Endy, Timothy P.
Ennis, Francis A.
Kalayanarooj, Siripen
Rothman, Alan L.
UMass Chan Affiliations
Department of Medicine, Division of Infectious Diseases and ImmunologyDocument Type
Journal ArticlePublication Date
2012-12-19
Metadata
Show full item recordAbstract
BACKGROUND: Infection with dengue viruses (DENV) causes a wide range of manifestations from asymptomatic infection to a febrile illness called dengue fever (DF), to dengue hemorrhagic fever (DHF). The in vivo targets of DENV and the relation between the viral burden in these cells and disease severity are not known. METHOD: The levels of positive and negative strand viral RNA in peripheral blood monocytes, T/NK cells, and B cells and in plasma of DF and DHF cases were measured by quantitative RT-PCR. RESULTS: Positive strand viral RNA was detected in monocytes, T/NK cells and B cells with the highest amounts found in B cells. Viral RNA levels in CD14+ cells and plasma were significantly higher in DHF compared to DF, and in cases with a secondary infection compared to those undergoing a primary infection. The distribution of viral RNA among cell subpopulations was similar in DF and DHF cases. Small amounts of negative strand RNA were found in a few cases only. The severity of plasma leakage correlated with viral RNA levels in plasma and in CD14+ cells. CONCLUSIONS: B cells were the principal cells containing DENV RNA in peripheral blood, but overall there was little active DENV RNA replication detectable in peripheral blood mononuclear cells (PBMC). Secondary infection and DHF were associated with higher viral burden in PBMC populations, especially CD14+ monocytes, suggesting that viral infection of these cells may be involved in disease pathogenesis.Source
PLoS One. 2012;7(12):e51335. doi: 10.1371/journal.pone.0051335. Epub 2012 Dec 19. Link to article on publisher's siteDOI
10.1371/journal.pone.0051335Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39584PubMed ID
23284680Related Resources
Link to Article in PubMedRights
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Distribution License
http://creativecommons.org/publicdomain/zero/1.0/ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0051335
Scopus Count
Collections
Except where otherwise noted, this item's license is described as <p>This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.</p>