PubMed ID
18830408
UMMS Affiliation
Department of Medicine, Division of Infectious Diseases and Immunology; Center for Infectious Disease and Vaccine Research
Date
10-2-2008
Document Type
Article
Subjects
Animals; B-Lymphocytes; CD8-Positive T-Lymphocytes; Mice; Mice, Inbred C57BL; Vaccines, Synthetic; Vaccinia virus; Viral Proteins; Viral Vaccines
Disciplines
Immunology and Infectious Disease | Life Sciences | Medicine and Health Sciences
Abstract
BACKGROUND: Vaccinia viruses have been used as a model for viral disease and as a protective live vaccine.
METHODOLOGY AND PRINCIPAL FINDINGS: We investigated the immunogenicity of an attenuated strain of vaccinia virus engineered to inactivate the N1L gene (vGK5). Using the intranasal route, this recombinant virus was 2 logs less virulent compared to the wildtype VACV-WR. Infection by the intranasal, intraperitoneal, and tail scarification routes resulted in the robust induction of cytolytic virus-specific CD8 T cells in the spleens and the lungs. VACV-specific antibodies were also detected in the sera of mice infected 3-5 months prior with the attenuated vGK5 virus. Finally, mice immunized with vGK5 were significantly protected when challenged with a lethal dose of VACV-WR.
CONCLUSIONS: These results indicate that the attenuated vGK5 virus protects against subsequent infection and suggest that the N1L protein limits the strength of the early antiviral CD8 T cell response following respiratory infection.
Comments
Citation:Mathew A, O'Bryan J, Marshall W, Kotwal GJ, Terajima M, et al. (2008) Robust Intrapulmonary CD8 T Cell Responses and Protection with an Attenuated N1L Deleted Vaccinia Virus. PLoS ONE 3(10): e3323. doi:10.1371/journal.pone.0003323. Link to article on publisher's site
Copyright: © 2008 Mathew et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.