Department of Biochemistry and Molecular Pharmacology
MicroRNAs; RNA-Induced Silencing Complex
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Life Sciences | Medicine and Health Sciences
BACKGROUND: MicroRNA (miRNA) are diverse in sequence and have a single known sequence bias: they tend to start with uridine (U).
RESULTS: Our analyses of fly, worm and mouse miRNA sequence data reveal that the 5'-U is recognized after miRNA production. Only one of the two strands can be assembled into Argonaute protein from a single miRNA/miRNA* molecule: in fly embryo lysate, a 5'-U promotes miRNA loading while decreasing the loading of the miRNA*.
CONCLUSION: We suggest that recognition of the 5'-U enhances Argonaute loading by a mechanism distinct from its contribution to weakening base pairing at the 5'-end of the prospective miRNA and, as recently proposed in Arabidopsis and in humans, that it improves miRNA precision by excluding incorrectly processed molecules bearing other 5'-nt.
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Citation: Silence. 2011 Jun 7;2:4. Link to article on publisher's site
Seitz, Herve; Tushir, Jogender S.; and Zamore, Phillip D., "A 5'-uridine Amplifies miRNA/miRNA* Asymmetry in Drosophila by Promoting RNA-induced Silencing Complex Formation" (2011). Open Access Articles. Paper 2289.