Modulation of HIV-1 macrophage-tropism among R5 envelopes occurs before detection of neutralizing antibodies
UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyProgram in Molecular Medicine
Document Type
Journal ArticlePublication Date
2010-05-29Keywords
Antibodies, NeutralizingCells, Cultured
HIV Antibodies
HIV Infections
HIV-1
Humans
London
Macrophages
*Viral Tropism
env Gene Products, Human Immunodeficiency Virus
Life Sciences
Medicine and Health Sciences
Virology
Metadata
Show full item recordAbstract
HIV-1 R5 viruses vary widely in their capacity to infect primary macrophages. R5 macrophage-tropism is associated with an increased envelope:CD4 affinity that partly results from an increased exposure of CD4 contact residues on gp120 and allows the use of low levels of CD4 for infection. The selective pressures in vivo that modulate R5 macrophage-tropism are not understood. It is possible that different R5 variants adapt for replication in either T-cells (high CD4) or in macrophages (low CD4). However, other selective pressures in vivo (e.g. neutralizing antibodies) may also impact R5 tropism. Here, we measured macrophage infectivity conferred by gp120 sequences amplified sequentially from subjects in London followed from the acute stage of infection. We report wide variation in the capacity of these envelopes to confer macrophage infection in the complete absence of both autologous and heterologous neutralizing antibodies. Our data show that the variation in macrophage tropism observed at early times cannot have been influenced by neutralizing antibodies.Source
Retrovirology. 2010 May 27;7:48. Link to article on publisher's siteDOI
10.1186/1742-4690-7-48Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39432PubMed ID
20507591Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1186/1742-4690-7-48