Title

F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA damage

UMMS Affiliation

Program in Molecular Medicine; Program in Gene Function and Expression

Date

5-5-2009

Document Type

Article

Subjects

Acetylcysteine; Cell Cycle Proteins; Cell Line, Tumor; Cyclin D1; Cysteine Proteinase Inhibitors; DNA Damage; DNA-Binding Proteins; F-Box Proteins; G1 Phase; Humans; Melanoma; Proteasome Endopeptidase Complex; Protein-Serine-Threonine Kinases; Transcriptional Activation; Tumor Suppressor Proteins; Ubiquitination

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

In response to DNA damage, eukaryotic cells initiate a complex signalling pathway, termed the DNA damage response (DDR), which coordinates cell cycle arrest with DNA repair. Studies have shown that oncogene-induced senescence, which provides a barrier to tumour development, involves activation of the DDR. Using a genome-wide RNA interference (RNAi) screen, we have identified 17 factors required for oncogenic BRAF to induce senescence in primary fibroblasts and melanocytes. One of these factors is an F-box protein, FBXO31, a candidate tumour suppressor encoded in 16q24.3, a region in which there is loss of heterozygosity in breast, ovarian, hepatocellular and prostate cancers. Here we study the cellular role of FBXO31, identify its target substrate and determine the basis for its growth inhibitory activity. We show that ectopic expression of FBXO31 acts through a proteasome-directed pathway to mediate the degradation of cyclin D1, an important regulator of progression from G1 to S phase, resulting in arrest in G1. Cyclin D1 degradation results from a direct interaction with FBXO31 and is dependent on the F-box motif of FBXO31 and phosphorylation of cyclin D1 at Thr 286, which is known to be required for cyclin D1 proteolysis. The involvement of the DDR in oncogene-induced senescence prompted us to investigate the role of FBXO31 in DNA repair. We find that DNA damage induced by gamma-irradiation results in increased FBXO31 levels, which requires phosphorylation of FBXO31 by the DDR-initiating kinase ATM. RNAi-mediated knockdown of FBXO31 prevents cells from undergoing efficient arrest in G1 after gamma-irradiation and markedly increases sensitivity to DNA damage. Finally, we show that a variety of DNA damaging agents all result in a large increase in FBXO31 levels, indicating that induction of FBXO31 is a general response to genotoxic stress. Our results reveal FBXO31 as a regulator of the G1/S transition that is specifically required for DNA damage-induced growth arrest.

Rights and Permissions

Citation: Nature. 2009 Jun 4;459(7247):722-5. Epub 2009 May 3. Link to article on publisher's site

DOI of Published Version

10.1038/nature08011

Related Resources

Link to Article in PubMed

Journal Title

Nature

PubMed ID

19412162