Title

Insulin receptor substrate-2 regulates aerobic glycolysis in mouse mammary tumor cells via glucose transporter 1

UMMS Affiliation

Department of Cancer Biology

Date

12-6-2008

Document Type

Article

Subjects

1-Phosphatidylinositol 3-Kinase; Aerobiosis; Animals; Breast Neoplasms; Carrier Proteins; Cell Line; Cell Membrane; Gene Expression Regulation, Neoplastic; Glucose; Glucose Transporter Type 1; Insulin Receptor Substrate Proteins; Mice; Mice, Knockout; Neoplasm Invasiveness; Neoplasm Metastasis; Phosphotransferases (Alcohol Group Acceptor); Protein Binding; Signal Transduction

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The insulin receptor substrate (IRS) proteins are cytoplasmic adaptor molecules that function as signaling intermediates downstream of activated cell surface receptors. Based on data implicating IRS-2 but not IRS-1 in breast cancer invasion, survival, and metastasis, we assessed the contribution of IRS-1 and IRS-2 to aerobic glycolysis, which is known to impact tumor growth and progression. For this purpose, we used tumor cell lines derived from transgenic mice that express the polyoma virus middle T antigen (PyV-MT) in the mammary gland and that are wild-type (WT) or null for either Irs-1 (Irs-1-/-) or Irs-2 (Irs-2-/-). Aerobic glycolysis, as assessed by the rate of lactic acid production and glucose consumption, was diminished significantly in Irs-2-/- cells when compared with WT and Irs-1-/- cells. Expression of exogenous Irs-2 in Irs-2-/- cells restored the rate of glycolysis to that observed in WT cells. The transcription factor FoxO1 does not appear to be involved in Irs-2-mediated glycolysis. However, Irs-2 does regulate the surface expression of glucose transporter 1 (Glut1) as assessed by flow cytometry using a Glut1-specific ligand. Suppression of Glut1 expression inhibits Irs-2-dependent invasion, which links glycolysis to mammary tumor progression. Irs-2 was shown to be important for mammalian target of rapamycin (mTor) activation, and Irs-2-dependent regulation of Glut1 surface expression is rapamycin-sensitive. Collectively, our data indicate that Irs-2, but not Irs-1, promotes invasion by sustaining the aerobic glycolysis of mouse mammary tumor cells and that it does so by regulating the mTor-dependent surface expression of Glut1.

Rights and Permissions

Citation: J Biol Chem. 2009 Jan 23;284(4):2031-7. Epub 2008 Dec 4. Link to article on publisher's site

DOI of Published Version

10.1074/jbc.M804776200

Related Resources

Link to Article in PubMed

Journal Title

The Journal of biological chemistry

PubMed ID

19056742