Co-stimulation of the bone-related Runx2 P1 promoter in mesenchymal cells by SP1 and ETS transcription factors at polymorphic purine-rich DNA sequences (Y-repeats)
Department of Cell Biology
3T3 Cells; Animals; Base Sequence; Cell Differentiation; Chromatin Immunoprecipitation; Core Binding Factor Alpha 1 Subunit; DNA Primers; Electrophoretic Mobility Shift Assay; Gene Expression Profiling; Gene Knockdown Techniques; Genes, Reporter; Luciferases; Mice; Osteoblasts; *Polymorphism, Genetic; *Promoter Regions, Genetic; Proto-Oncogene Protein c-ets-1; RNA Interference; *Repetitive Sequences, Nucleic Acid; Sp1 Transcription Factor
Life Sciences | Medicine and Health Sciences
Transcriptional control of Runx2 gene expression through two alternative promoters (P1 and P2) is critical for the execution of its function as an osteogenic cell fate determining factor. In all vertebrates examined to date, the bone related P1 promoter contains a purine-rich region (-303 to -128 bp in the rat) that separates two regulatory domains. The length of this region differs dramatically between species even within the same order. Using deletion analysis, we show that part of this purine-rich region (-200 to -128) containing a duplicated element (Y-repeat) positively regulates Runx2 P1 transcription. Electrophoretic mobility assays and chromatin immunoprecipitations reveal that Y-repeat binds at least two different classes of transcription factors related to GC box binding proteins (e.g. SP1 and SP7/Osterix) and ETS-like factors (e.g. ETS1 and ELK1). Forced expression of SP1 increases Runx2 P1 promoter activity through the Y-repeats, and small interfering RNA depletion of SP1 decreases Runx2 expression. Similarly, exogenous expression of wild type ELK1, but not a defective mutant that cannot be phosphorylated, enhances Runx2 gene expression. SP1 is most abundant in proliferating cells, and ELK1 is most abundant in postconfluent cells; during MC3T3-E1 osteoblast differentiation, both proteins are transiently co-expressed when Runx2 expression is enhanced. Taken together, our data suggest that basal Runx2 gene transcription is regulated by dynamic interactions between SP1 and ETS-like factors during progression of osteogenesis.
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Citation: J Biol Chem. 2009 Jan 30;284(5):3125-35. Epub 2008 Nov 18. Link to article on publisher's site