Title

nhl-2 Modulates microRNA activity in Caenorhabditis elegans

UMMS Affiliation

Program in Molecular Medicine

Publication Date

3-10-2009

Document Type

Article

Subjects

Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Carrier Proteins; Cytoplasmic Granules; Homeodomain Proteins; MicroRNAs; RNA Nucleotidyltransferases; RNA, Helminth; RNA-Induced Silencing Complex

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

TRIM-NHL proteins represent a large class of metazoan proteins implicated in development and disease. We demonstrate that a C. elegans TRIM-NHL protein, NHL-2, functions as a cofactor for the microRNA-induced silencing complex (miRISC) and thereby enhances the posttranscriptional repression of several genetically verified microRNA targets, including hbl-1 and let-60/Ras (by the let-7 family of microRNAs) and cog-1 (by the lsy-6 microRNA). NHL-2 is localized to cytoplasmic P-bodies and physically associates with the P-body protein CGH-1 and the core miRISC components ALG-1/2 and AIN-1. nhl-2 and cgh-1 mutations compromise the repression of microRNA targets in vivo but do not affect microRNA biogenesis, indicating a role for an NHL-2:CGH-1 complex in the effector phase of miRISC activity. We propose that the NHL-2:CGH-1 complex functions in association with mature miRISC to modulate the efficacy of microRNA:target interactions in response to physiological and developmental signals, thereby ensuring the robustness of genetic regulatory pathways regulated by microRNAs.

Rights and Permissions

Citation: Cell. 2009 Mar 6;136(5):926-38. Link to article on publisher's site

DOI of Published Version

10.1016/j.cell.2009.01.053

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Cell

PubMed ID

19269369