Title

The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters

UMMS Affiliation

Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology

Publication Date

9-8-2009

Document Type

Article

Subjects

Animals; Chromatin Immunoprecipitation; Chromosomal Proteins, Non-Histone; *DNA Transposable Elements; Drosophila Proteins; Drosophila melanogaster; *Gene Silencing; Heterochromatin; RNA, Small Interfering; Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Piwi-interacting RNAs (piRNAs) silence transposons and maintain genome integrity during germline development. In Drosophila, transposon-rich heterochromatic clusters encode piRNAs either on both genomic strands (dual-strand clusters) or predominantly one genomic strand (uni-strand clusters). Primary piRNAs derived from these clusters are proposed to drive a ping-pong amplification cycle catalyzed by proteins that localize to the perinuclear nuage. We show that the HP1 homolog Rhino is required for nuage organization, transposon silencing, and ping-pong amplification of piRNAs. rhi mutations virtually eliminate piRNAs from the dual-strand clusters and block production of putative precursor RNAs from both strands of the major 42AB dual-strand cluster, but not of transcripts or piRNAs from the uni-strand clusters. Furthermore, Rhino protein associates with the 42AB dual-strand cluster,but does not bind to uni-strand cluster 2 or flamenco. Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle.

Rights and Permissions

Citation: Cell. 2009 Sep 18;138(6):1137-49. Epub 2009 Sep 3. Link to article on publisher's site

DOI of Published Version

10.1016/j.cell.2009.07.014

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Cell

PubMed ID

19732946