UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

9-15-2009

Document Type

Article

Subjects

Genes, cdc; Protein-Serine-Threonine Kinases; S Phase; Protein-Tyrosine Kinases; Schizosaccharomyces pombe Proteins; DNA-Binding Proteins; DNA Replication; Transcription Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Checkpoint activation during S phase modulates transcription. In response to replication arrest, the fission yeast Cds1 checkpoint kinase maintains the normal S-phase transcriptional program by regulating MBF, the S-phase transcription factor. We show that similar regulation occurs in response to DNA damage during S-phase. We test the relative contributions to replication-stress resistance of transcriptional regulation and the two other major checkpoint functions: cell-cycle arrest and fork stabilization. We show that, although transcriptional regulation provides only modest resistance relative to fork stabilization, it contributes significantly to cell survival. Finally, we investigate the roles of two specific transcripts: mik1 and mrc1. These results demonstrate the general importance of checkpoint regulation of G1/S transcription in response to replicative stress and elucidate the specific roles of Mik1 and Mrc1 in the checkpoint.

Rights and Permissions

Citation: PLoS One. 2009 Sep 11;4(9):e6944. Link to article on publisher's site

DOI of Published Version

10.1371/journal.pone.0006944

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

PloS one

PubMed ID

19750219

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