The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
Journal ArticlePublication Date
2009-09-15Keywords
Genes, cdcProtein-Serine-Threonine Kinases
S Phase
Protein-Tyrosine Kinases
Schizosaccharomyces pombe Proteins
DNA-Binding Proteins
DNA Replication
Transcription Factors
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Checkpoint activation during S phase modulates transcription. In response to replication arrest, the fission yeast Cds1 checkpoint kinase maintains the normal S-phase transcriptional program by regulating MBF, the S-phase transcription factor. We show that similar regulation occurs in response to DNA damage during S-phase. We test the relative contributions to replication-stress resistance of transcriptional regulation and the two other major checkpoint functions: cell-cycle arrest and fork stabilization. We show that, although transcriptional regulation provides only modest resistance relative to fork stabilization, it contributes significantly to cell survival. Finally, we investigate the roles of two specific transcripts: mik1 and mrc1. These results demonstrate the general importance of checkpoint regulation of G1/S transcription in response to replicative stress and elucidate the specific roles of Mik1 and Mrc1 in the checkpoint.Source
PLoS One. 2009 Sep 11;4(9):e6944. Link to article on publisher's siteDOI
10.1371/journal.pone.0006944Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39278PubMed ID
19750219Related Resources
Link to Article in PubMedRights
Copyright: © 2009 Dutta, Rhind. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0006944