Title

Three-dimensional structure of vertebrate cardiac muscle myosin filaments

UMMS Affiliation

Department of Cell Biology

Date

2-7-2008

Document Type

Article

Subjects

Animals; Cardiac Myosins; Imaging, Three-Dimensional; Mice; Microscopy, Electron; Models, Molecular; Muscle Proteins; Myocardium; Protein Kinases; Protein Structure, Tertiary

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Contraction of the heart results from interaction of the myosin and actin filaments. Cardiac myosin filaments consist of the molecular motor myosin II, the sarcomeric template protein, titin, and the cardiac modulatory protein, myosin binding protein C (MyBP-C). Inherited hypertrophic cardiomyopathy (HCM) is a disease caused mainly by mutations in these proteins. The structure of cardiac myosin filaments and the alterations caused by HCM mutations are unknown. We have used electron microscopy and image analysis to determine the three-dimensional structure of myosin filaments from wild-type mouse cardiac muscle and from a MyBP-C knockout model for HCM. Three-dimensional reconstruction of the wild-type filament reveals the conformation of the myosin heads and the organization of titin and MyBP-C at 4 nm resolution. Myosin heads appear to interact with each other intramolecularly, as in off-state smooth muscle myosin [Wendt T, Taylor D, Trybus KM, Taylor K (2001) Proc Natl Acad Sci USA 98:4361-4366], suggesting that all relaxed muscle myosin IIs may adopt this conformation. Titin domains run in an elongated strand along the filament surface, where they appear to interact with part of MyBP-C and with the myosin backbone. In the knockout filament, some of the myosin head interactions are disrupted, suggesting that MyBP-C is important for normal relaxation of the filament. These observations provide key insights into the role of the myosin filament in cardiac contraction, assembly, and disease. The techniques we have developed should be useful in studying the structural basis of other myosin-related HCM diseases.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2386-90. Epub 2008 Feb 5. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

18252826