Dynein-dynactin complex is essential for dendritic restriction of TM1-containing Drosophila Dscam
Student Authors
Shun-Jen (Jacob) S. YangAcademic Program
NeuroscienceUMass Chan Affiliations
Graduate School of Biomedical Sciences, Neuroscience ProgramLee Lab
Neurobiology
Document Type
Journal ArticlePublication Date
2008-10-24Keywords
AnimalsAnimals, Genetically Modified
Dendrites
Drosophila
Drosophila Proteins
Dynein ATPase
Membrane Proteins
Microtubule-Associated Proteins
Models, Biological
Mosaicism
Multiprotein Complexes
Organ Specificity
Protein Binding
Protein Isoforms
Protein Structure, Tertiary
Protein Transport
Neuroscience and Neurobiology
Metadata
Show full item recordAbstract
BACKGROUND: Many membrane proteins, including Drosophila Dscam, are enriched in dendrites or axons within neurons. However, little is known about how the differential distribution is established and maintained. METHODOLOGY/PRINCIPAL FINDINGS: Here we investigated the mechanisms underlying the dendritic targeting of Dscam[TM1]. Through forward genetic mosaic screens and by silencing specific genes via targeted RNAi, we found that several genes, encoding various components of the dynein-dynactin complex, are required for restricting Dscam[TM1] to the mushroom body dendrites. In contrast, compromising dynein/dynactin function did not affect dendritic targeting of two other dendritic markers, Nod and Rdl. Tracing newly synthesized Dscam[TM1] further revealed that compromising dynein/dynactin function did not affect the initial dendritic targeting of Dscam[TM1], but disrupted the maintenance of its restriction to dendrites. CONCLUSIONS/SIGNIFICANCE: The results of this study suggest multiple mechanisms of dendritic protein targeting. Notably, dynein-dynactin plays a role in excluding dendritic Dscam, but not Rdl, from axons by retrograde transport.Source
PLoS One. 2008;3(10):e3504. Epub 2008 Oct 23. Link to article on publisher's siteDOI
10.1371/journal.pone.0003504Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39264PubMed ID
18946501Notes
Co-author Jacob S. Yang is a student in the Neuroscience program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.
Related Resources
Link to Article in PubMedRights
Copyright: © 2008 Yang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0003504
Scopus Count
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