Relative immunogenicity and protection potential of candidate Yersinia Pestis antigens against lethal mucosal plague challenge in Balb/C mice
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UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyLaboratory of Nucleic Acid Vaccines
Document Type
Journal ArticlePublication Date
2008-02-23Keywords
AnimalsAntibodies, Bacterial
Antigens, Viral
Bacterial Proteins
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Female
Immunity, Mucosal
Immunoglobulin G
Mice
Mice, Inbred BALB C
Plague
Plague Vaccine
Plasminogen Activators
Protein Engineering
Vaccines, DNA
Vaccines, Synthetic
Yersinia pestis
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Yersinia Pestis outer proteins, plasminogen activator protease and Yop secretion protein F are necessary for the full virulence of Yesinia pestis and have been proposed as potential protective antigens for vaccines against plague. In the current study, we used DNA immunization as a tool to study the relative protective immunity of these proteins with a standardized intranasal challenge system in mice. While the natural full-length gene sequences for most of these Y. pestis proteins did not display a good level of protein expression in vitro when delivered by a DNA vaccine vector, the overall immunogenicity of these wild type gene DNA vaccines was low in eliciting antigen-specific antibody responses and gene sequence modifications improved both of these parameters. However, even modified YopD, YopO and YscF antigens were only able to partially protect immunized mice at various levels against lethal challenge with Y. pestis KIM 1001 strain while no protection was observed with either the YopB or Pla antigens. These results demonstrate that DNA immunization is effective in screening, optimizing and comparing optimal antigen designs and immunogenicity of candidate antigens for the development of a subunit-based plague vaccine.Source
Vaccine. 2008 Mar 20;26(13):1664-74. Epub 2008 Feb 4. Link to article on publisher's site
DOI
10.1016/j.vaccine.2008.01.024Permanent Link to this Item
http://hdl.handle.net/20.500.14038/39253PubMed ID
18291562Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/j.vaccine.2008.01.024