Class II major histocompatibility complex tetramer staining: progress, problems, and prospects
Department of Biochemistry and Molecular Pharmacology; Department of Pathology
Antibody Affinity; CD4-Positive T-Lymphocytes; Flow Cytometry; Histocompatibility Antigens Class II; Humans; Staining and Labeling
Immunology and Infectious Disease
The use of major histocompatibility complex (MHC) tetramers in the detection and analysis of antigen-specific T cells has become more widespread since its introduction 11 years ago. Early challenges in the application of tetramer staining to CD4+ T cells centred around difficulties in the expression of various class II MHC allelic variants and the detection of low-frequency T cells in mixed populations. As many of the technical obstacles to class II MHC tetramer staining have been overcome, the focus has returned to uncertainties concerning how oligomer valency and T-cell receptor/MHC affinity affect tetramer binding. Such issues have become more important with an increase in the number of studies relying on direct ex vivo analysis of antigen-specific CD4+ T cells. In this review we discuss which problems in class II MHC tetramer staining have been solved to date, and which matters remain to be considered.
Rights and Permissions
Citation: Immunology. 2008 Mar;123(3):305-13. Link to article on publisher's site
DOI of Published Version
Vollers, Sabrina S. and Stern, Lawrence J., "Class II major histocompatibility complex tetramer staining: progress, problems, and prospects" (2008). Open Access Articles. 2052.