Title

Class II major histocompatibility complex tetramer staining: progress, problems, and prospects

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Department of Pathology

Date

2-7-2008

Document Type

Article

Subjects

Antibody Affinity; CD4-Positive T-Lymphocytes; Flow Cytometry; Histocompatibility Antigens Class II; Humans; Staining and Labeling

Disciplines

Immunology and Infectious Disease

Abstract

The use of major histocompatibility complex (MHC) tetramers in the detection and analysis of antigen-specific T cells has become more widespread since its introduction 11 years ago. Early challenges in the application of tetramer staining to CD4+ T cells centred around difficulties in the expression of various class II MHC allelic variants and the detection of low-frequency T cells in mixed populations. As many of the technical obstacles to class II MHC tetramer staining have been overcome, the focus has returned to uncertainties concerning how oligomer valency and T-cell receptor/MHC affinity affect tetramer binding. Such issues have become more important with an increase in the number of studies relying on direct ex vivo analysis of antigen-specific CD4+ T cells. In this review we discuss which problems in class II MHC tetramer staining have been solved to date, and which matters remain to be considered.

Rights and Permissions

Citation: Immunology. 2008 Mar;123(3):305-13. Link to article on publisher's site

DOI of Published Version

10.1111/j.1365-2567.2007.02801.x

Comments

Co-author Sabrina Vollers is a student in the Biochemistry & Molecular Pharmacology program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources

Link to Article in PubMed

Journal Title

Immunology

PubMed ID

18251991