Title

A polycystin-1 controls postcopulatory reproductive selection in mice

UMMS Affiliation

Department of Cell Biology

Publication Date

6-20-2008

Document Type

Article

Subjects

Acrosome; Animals; Copulation; Female; Fertilization; Genotype; Male; Mating Preference, Animal; Mice; Mice, Transgenic; Receptors, Cell Surface; Reproduction; *Sperm Capacitation; Sperm Motility; TRPP Cation Channels

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

Pkdrej, a member of the polycystin-1 gene family, is expressed only in the male germ line. Male mice that are homozygous for a targeted mutation in the Pkdrej allele (Pkdrej(tm/tm)) are fertile in unrestricted mating trials, but exhibit lower reproductive success when competing with wild-type males in sequential mating trials and in artificial insemination of mixed-sperm populations. Following mating, sperm from Pkdrej(tm/tm) mice require >2 h longer than those of wild-type males to be detected within the egg/cumulus complex in the oviduct. Sperm from mice of both genotypes are able to capacitate in vitro. However, one of the component processes of capacitation, the ability to undergo a zona pellucida-evoked acrosome reaction, develops more slowly in sperm from Pkdrej(tm/tm) animals than in sperm from wild-type males. In contrast, a second component process of capacitation, the transition to hyperactivated flagellar motility, develops with a similar time course in both genotypes. These two behavioral consequences of capacitation, exocytotic competence and altered motility, are therefore differentially regulated. These data suggest that Pkdrej controls the timing of fertilization in vivo through effects on sperm transport and exocytotic competence and is a factor in postcopulatory sexual selection.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8661-6. Epub 2008 Jun 18. Link to article on publisher's site

DOI of Published Version

10.1073/pnas.0800603105

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Proceedings of the National Academy of Sciences of the United States of America

PubMed ID

18562295