Title

Trp53 deletion stimulates the formation of metastatic pancreatic tumors

UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine

Publication Date

3-4-2008

Document Type

Article

Subjects

Animals; Antigens, Polyomavirus Transforming; Chickens; Cyclin-Dependent Kinase Inhibitor p16; *Gene Deletion; Injections; Mice; Neoplasm Metastasis; Pancreatic Elastase; Pancreatic Neoplasms; Tumor Suppressor Protein p53

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The presence of distant metastases is a common finding on diagnosis of pancreatic cancer; however, the mechanisms underlying the dissemination of this tumor type remain poorly understood. Loss of the p53 tumor suppressor protein has been associated with tumor progression and metastasis in several tumor types including pancreatic ductal adenocarcinoma. Here, we describe the generation of a progressive and metastatic pancreatic cancer mouse model after the somatic and sporadic delivery of avian retroviruses encoding the mouse polyoma virus middle T antigen to elastase-tv-a transgenic mice with a pancreas-specific deletion of the Trp53 tumor suppressor locus. In this model, the tumors metastasize most frequently to the liver, consistent with human pancreatic carcinomas. Analysis of metastatic lesions demonstrated that concomitant loss of the Ink4a/Arf locus was not required for metastasis; however, pancreas-specific deletion of a single Ink4a/Arf allele cooperated with Trp53 deletion in a haploinsufficient manner to accelerate tumor development. Thus, our findings illustrate the potential role of p53 loss of function in pancreatic tumor progression, demonstrate the feasibility of modeling pancreatic cancer metastasis after somatic and sporadic oncogene activation, and indicate that our model may provide a useful experimental system for investigation of the molecular mechanisms underlying pancreatic cancer progression and metastasis.

Rights and Permissions

Citation: Am J Pathol. 2008 Apr;172(4):1081-7. Epub 2008 Feb 29. Link to article on publisher's site

DOI of Published Version

10.2353/ajpath.2008.070778

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

The American journal of pathology

PubMed ID

18310506