UMMS Affiliation

Center for Infectious Disease and Vaccine Research

Date

10-3-2008

Document Type

Article

Subjects

Animals; B-Lymphocytes; CD8-Positive T-Lymphocytes; Mice; Mice, Inbred C57BL; Vaccines, Synthetic; Vaccinia virus; Viral Proteins; Viral Vaccines

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

BACKGROUND: Vaccinia viruses have been used as a model for viral disease and as a protective live vaccine. METHODOLOGY AND PRINCIPAL FINDINGS: We investigated the immunogenicity of an attenuated strain of vaccinia virus engineered to inactivate the N1L gene (vGK5). Using the intranasal route, this recombinant virus was 2 logs less virulent compared to the wildtype VACV-WR. Infection by the intranasal, intraperitoneal, and tail scarification routes resulted in the robust induction of cytolytic virus-specific CD8 T cells in the spleens and the lungs. VACV-specific antibodies were also detected in the sera of mice infected 3-5 months prior with the attenuated vGK5 virus. Finally, mice immunized with vGK5 were significantly protected when challenged with a lethal dose of VACV-WR. CONCLUSIONS: These results indicate that the attenuated vGK5 virus protects against subsequent infection and suggest that the N1L protein limits the strength of the early antiviral CD8 T cell response following respiratory infection.

Rights and Permissions

Citation: PLoS One. 2008 Oct 2;3(10):e3323. Link to article on publisher's site

DOI of Published Version

10.1371/journal.pone.0003323

Related Resources

Link to Article in PubMed

Journal Title

PloS one

PubMed ID

18830408

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